James Shea is in goal, with Alan Sheehan eligible to captain the side from the heart of defence despite serving a three-match league and FA Cup suspension, as this competition has its own discipline rules.
Pelly-Ruddock Mpanzu in the middle of midfield is the only player included who started at Newcastle on Saturday, while Harry Cornick and Luke Gambin – both who appeared as substitutes at St James’ Park – start in attack.
Peterborough include former Hatters striker Jack Marriott, who left the Town in the summer to move to London Road.
A reminder that if the scores are level at the end of 90 minutes tonight, there will be no extra-time or replay. The tie will go straight to a penalty shoot-out to determine which team goes through to the quarter-final stage.
As was the case in the games against Tottenham Hotspur U21s and Barnet, when the Town won both to earn group stage bonus points, the shoot-out will follow the new ABBA format being trialled in EFL competitions this season rather than the traditional ABAB sequence.
TOWN: Shea, Musonda, Sheehan ©, Famewo, Stacey, Mpanzu, D’Ath, Senior, Gambin, Cornick, Cook. Subs: Justin, Potts, O Lee, Shinnie, McQuoid, Cotter, Isted (GK)
POSH: Bond, Shephard, Hughes, Tafazolli, Baldwin ©, Lloyd, Maddison, Marriott, Morias, Da Silva Lopes, Grant. Subs: Penny, Kanu, Doughty, Anderson, O’Malley (GK), Freestone, Borg
REFEREE: Anthony Coggins