Henri Lansbury comes into midfield alongside Jordan Clark with Carlos Mendes Gomes ahead of them.
Cameron Jerome starts with Elijah Adebayo up-front as the gaffer names a strong starting line-up as the Hatters bid to book their place in round four of the competition.
There will be no replays in the third round and in the event of a draw at full-time, extra-time and penalties will be played.
Luton: Shea; Bree, Burke, Bradley (C), Naismith, Bell; Lansbury, Clark, Mendes Gomes; Adebayo, Jerome.
Substitutes: Sluga, Potts, Rea, Berry, Hylton, Mpanzu, Campbell, Onyedinma, Osho.
Harrogate: Oxley; Fallowfield, Falkingham (C), McArdle, Page; Diarra, Kerry, Sheron; Muldoon, Thomson; Armstrong.
Substitutes: Burrell, Cracknell, Austerfield, Hall.