New signing Alfie Doughty will play the first 45 minutes, so too will trialist Baba Fernandes who is without a club after leaving Nottingham Forest this summer.
The centre-back starts with Gabe Osho and Dan Potts in defence, whilst Cameron Jerome and Admiral Muskwe start together up-front.
Matt Macey and Cauley Woodrow are both set to be involved in the second period as well as Development squad players Josh Williams and Casey Pettit.
Luton (first half): Isted; Fernandes, Osho, Potts; Lansbury; Thorpe, Clark, Mendes Gomes, Doughty; Jerome, Muskwe.
Luton (second half): Macey; Burke, Lockyer, Williams; Bree, Pereira, Pettit, Onyedinma; Woodrow; Adebayo, Cornick.
Hitchin: Horlock (C), Green, Black, Coldicott-Stevens, Georgiou, Jones, Popoola, Gleeson, Tearle, Dasilva, Allotey.