Pelly-Ruddock Mpanzu makes his first league start since April after he was a second-half substitute on Saturday afternoon.
Captain Sonny Bradley returns to the starting line-up, joining Tom Lockyer and Dan Potts in the heart of defence, with James Bree and Amari'i Bell occupying the width.
The third and final change is up front, with Harry Cornick brought into the attack ahead of top scorer Carlton Morris, who is amongst the substitutes.
Luton Town: Horvath; Bree, Lockyer, Bradley (C), Potts, Bell; Mpanzu, Campbell, Doughty; Adebayo, Cornick.
Substitutes: Isted, Berry, Morris, Watson, Freeman, Jerome, Pettit.
Reading: Lumley; Yiadom, Holmes, Rahman; Mbengue, Ejaria, Hendrick, Hoilett; Ince, Meite; Carroll.
Substitutes: Bouzanis, Long, Joao, Guinness-Walker, Fornah, Abbey, Senga-Ngoyi.
Referee: John Brooks