Ethan Horvath has recovered from illness that ruled him out of the midweek defeat against Stoke City.
Alfie Doughty comes back into the starting line-up and makes his fifth league start, replacing Harry Cornick who is amongst the substitutes.
Development Squad left-back Josh Williams joins Casey Pettit on the bench with Under 18 Head Coach Alan McCormack overlooking from the sideline, joined by Alex Lawless and Adrian Forbes.
Luton Town: Horvath; Doughty, Bree, Osho, Lockyer (C), Bell; Mpanzu, Campbell, Clark; Adebayo, Morris.
Substitutes: Isted, Cornick, Berry, Watson, Freeman, Williams, Pettit.
Rotherham United: Johansson; Kioso, Peltier, Humphreys, Harding, Bramall; Rathbone, Barlaser, Lindsay, Wiles; Ogbene.
Substitutes: Vickers, Wood, High, Eaves, Norton-Cuffy, Washington, Odoffin.
Referee: Stephen Martin