After beating Harrogate Town and Cambridge United en route to their first fifth round match since 2013, the Hatters will also be looking for revenge following the 3-1 defeat against the Blues in the competition last season.
We encourage all supporters at Wednesday's game to stand and join the applause in the 31st minute as we remember Jamal Edwards MBE who sadly passed away last month.
The opposition:
After reaching the final last year, Chelsea will be looking to go a step further this time around to add to their trophy cabinet.
They added to their long list of success in February as they became world champions, beating Palmeiras 2-1 in the Club World Cup final thanks to an extra-time Kai Havertz goal, who months earlier scored the goal to win Chelsea their second Champions League trophy.
Last time we met:
The two sides met in the fourth round of the FA Cup last season and a Tammy Abraham hat-trick helped book their place in the following round on that occasion.
Jordan Clark had given Town hope when he pulled a goal back on the half-hour mark before Abraham sealed progression with his third goal in the second half in Frank Lampard's last game as Chelsea manager.
Luton, however, were victorious during their last meeting at Kenilworth Road against Chelsea in 1991, recording a 2-0 win on that day.
Follow the action:
Those not able to join us at Kenilworth Road on the night can tune into the BBC's match coverage from 7pm.
As usual, supporters can also listen to Simon Pitts' match commentary on iFollow Hatters and he will be joined by former Town midfielder Alex Lawless on co-commentary duties.
Ref watch
Referee: Peter Bankes
Assistant Referees: Neil Davies and James Mainwaring
Fourth Official: Graham Scott
The man in the middle for this fixture is Peter Bankes, who refereed the Town's win against Blackpool in December.
He has taken a total of 18 matches so far this term, dishing out 73 yellow cards and two reds in the process.