Barkley, who made 179 appearances for the Toffees after coming through the ranks, is joined in midfield by Sambi Lokonga, who returns to the squad after being rested for the FA Cup replay at Bolton.
Tim Krul keeps his place in goal, while Andros Townsend and Elijah Adebayo also start having been used as a substitutes at Bolton.
Manager Rob Edwards selects two goalkeepers in James Shea and Thomas Kaminski among the subs, as well as academy graduates Zack Nelson and Joe Johnson.
Town: Krul; Burke, Mengi, Bell, Doughty, Obgene; Lokonga, Barkley; Townsend, Morris, Adebayo.
Subs: Shea, Kaminski, Potts, Berry, Woodrow, Chong, Mpanzu, Johnson, Nelson.
Everton: Virginia, Patterson, Tarkowski, Onana, Calvert-Lewin, Harrison, Beto, Mykolenko, Branthwaite, Garner.
Subs: Pickford, Lonergan, Keane, Danjuma, Young, Godfrey, Chermiti, Hunt, Dobbin.
Referee: Simon Hooper